Purification of three different microbial beta-galactosidases by partitioning in aqueous two-phase systems
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چکیده
منابع مشابه
Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems
Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...
متن کاملsynthesis of platinum nanostructures in two phase system
چکیده پلاتین، فلزی نجیب، پایدار و گران قیمت با خاصیت کاتالیزوری زیاد است که کاربرد های صنعتی فراوانی دارد. کمپلکس های پلاتین(ii) به عنوان دارو های ضد سرطان شناخته شدند و در شیمی درمانی بیماران سرطانی کاربرد دارند. خاصیت کاتالیزوری و عملکرد گزینشی پلاتین مستقیماً به اندازه و- شکل ماده ی پلاتینی بستگی دارد. بعضی از نانو ذرات فلزی در سطح مشترک مایع- مایع سنتز شده اند، اما نانو ساختار های پلاتین ب...
Af\nity Partitioning in Aqueous Two-Phase Systems
for use in extracorporeal circuits, for the removal of toxic substances such as certain metabolites or antibodies from blood. For example, exogenous human serum amyloid P component, a substance associated with Alzheimer’s disease, has been removed from whole rat blood in an extracorporeal circulation system. This model system used a polyclonal antibody coupled to cellulose Sat-sheet membranes. ...
متن کاملPurification of recombinant green fluorescent protein by three-phase partitioning.
The technique of three-phase partitioning (TPP) was used to purify the green fluorescent protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert-butanol to precipitate proteins from their crude extracts. In the first round of TPP with 20% ammonium sulphate saturation at the ratio of crude to tert-butanol 1:1 (v/v), most of the GFP remains in the lower aqueous phase....
متن کاملrapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems
background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...
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ژورنال
عنوان ژورنال: Ciência e Tecnologia de Alimentos
سال: 1997
ISSN: 0101-2061
DOI: 10.1590/s0101-20611997000300005